Types Of Chromatography
What are the different types of chromatography? Chromatography is the term used to describe sets of laboratory equipment or techniques that are mainly used to separate mixtures. The mixture that is being separated is dissolved in some fluid then it is carried into a structure that is housing another material. Because the constituents are moved in different speeds, the mixture will then separate. But do you know that there are many different types of chromatography? They are actually classified into analytical and preparative. However, they are sub classified into many other different types of chromatography.
Absorption chromatography is the types of chromatography where the chemical mixtures are separated through the use of an absorbent bed. Depending on the components of the mixture, there are different speeds that are used to separate them. This is why there have been many other sub types of these types of chromatography. These sub types are ion exchange chromatography, column chromatography, planar chromatography, and affinity chromatography.
Partition chromatography, on the other hand, is among the types of chromatography wherein the components of the mixture are separated by a certain partition solute. This is composed of two solvents. One of these two solvents is immobilized by the process. This way, part of the substance is taken out through a filter paper.
Gel filtration chromatography is among the types of chromatography wherein molecules are separated basing on their size. The basis of this separation is called hydrodynamic molecules. The large molecules are not able to pass through a certain filer. These molecules are then washed out. The smaller molecules, on the other hand, are able to enter the pores. Therefore, they are much harder to elute.
Next, we have high performance liquid chromatography. These are types of chromatography that separates mixtures by idiosyncratic polarities. A pump is used to separate these polarities. Depending on the interactions, retention of the mixtures is able to occur. This depends on the interactions of both the stationary stage and the analytic stage.
Gas chromatography is among types of chromatography that makes use of cylinders where gas is stored. These gasses carry a solute. Most often, the solute used here is helium. There are three main sub types of these types of chromatography – gas/liquid chromatography, gas abruption chromatography, and capillary gas chromatography.
Among the types of chromatography, we have the reverse phase chromatography wherein the method is the opposite of the normal phase one. Here, the stationary stage is mostly made of hydrophobic compounds that attract the same compounds that are in the mobile stage. This way, the polarity separates the mixture into the order they are deemed to be separated. Lastly, we have the pyrolysis gas chromatography. This is done by decomposition by the use of thermal power. Here, the regular process is followed by the process of gas chromatography. After resistive heating, there will be inductive heating as well as isothermal heating will take place. Then there will be volatile fragments that will be present. These will separate the mixture into the types of separation that is needed and aimed.